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Home Antibody All anti-NECAB1 antibodies

Anti-NECAB1 TRUEMAB Antibody Clone OTI4G8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502546 NECAB1 mouse monoclonal antibody, clone OTI4G8 (formerly 4G8) 100ul $379 In Stock
LC411679 NECAB1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502546 Carrier-free (BSA/glycerol-free) NECAB1 mouse monoclonal antibody, clone OTI4G8 (formerly 4G8) 100ug $450 3-4 weeks
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WB(1)
FC(3)
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Also for NECAB1 (NM_022351)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NECAB1 (NP_071746) produced in HEK293T cell.
Clone NameClone OTI4G8 IsotypeIgG2a
Species ReactivityHuman Concentration0.59 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:2000, FLOW 1:100
Predicted MW Explanation 40.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens N-terminal EF-hand calcium binding protein 1 (NECAB1)
Alternative NameEFCBP1; STIP-1
Database LinkNP_071746
Entrez Gene 64168 Human
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NECAB1 (RC209704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NECAB1.
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HEK293T cells transfected with either RC209704 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NECAB1 antibody(TA502546), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-NECAB1 antibody(TA502546),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-NECAB1 antibody(TA502546),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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