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Anti-NDUFAF1 Antibody EPR2795


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against NDUFAF1 (clone EPR2795 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC414253) , 20ug Explanation
100ul $325 3-7 Days
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Also for NDUFAF1 (NM_016013)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human NDUFAF1 was used as an immunogen.
Clone NameEPR2795 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsFC: 1:50, ICC: 1:50 - 100, IHC: 1:100 - 250, IP: 1:50, WB: 1:10,000 - 50,000,
Predicted MW Explanation 38 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens NADH dehydrogenase (ubiquinone) complex I, assembly factor 1 (NDUFAF1), transcript variant 1
Alternative NameCGI-65; CGI65; CIA30
Database LinkNP_057097
Entrez Gene 51103 Human
FunctionNDUFAF1 (CIA30) is a chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex 1) (1). Complex I is the largest multiprotein enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). Little is known about the assembly process of the subunits into the mature complex, however, NDUFAF1 is one of two proteins found to be involved. NDUFAF1 expression is ubiquitous with a slightly higher expression in various heart tissues, kidney, lung and liver (2).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - NDUFAF1 antibody [EPR2795]; All lanes : Anti-NDUFAF1 antibody [EPR2795] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A549 cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 38 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NDUFAF1 antibody [EPR2795]; Paraffin embedded Human liver tissue labelled with TA307093 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - NDUFAF1 antibody [EPR2795]; Immunofluorescence analysis of HeLa cells labelled with TA307093 at 1/500 dilution.


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