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Anti-NDUFAF1 Antibody EPR2795
Also for NDUFAF1 (NM_016013)
|A synthetic peptide corresponding to residues in human NDUFAF1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||FC: 1:50, ICC: 1:50 - 100, IHC: 1:100 - 250, IP: 1:50, WB: 1:10,000 - 50,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens NADH dehydrogenase (ubiquinone) complex I, assembly factor 1 (NDUFAF1), transcript variant 1|
|CGI-65; CGI65; CIA30|
|NDUFAF1 (CIA30) is a chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex 1) (1). Complex I is the largest multiprotein enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). Little is known about the assembly process of the subunits into the mature complex, however, NDUFAF1 is one of two proteins found to be involved. NDUFAF1 expression is ubiquitous with a slightly higher expression in various heart tissues, kidney, lung and liver (2). |
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Western blot - NDUFAF1 antibody [EPR2795]; All lanes : Anti-NDUFAF1 antibody [EPR2795] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A549 cell lysate.Lane 4 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 38 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NDUFAF1 antibody [EPR2795]; Paraffin embedded Human liver tissue labelled with ab92454 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - NDUFAF1 antibody [EPR2795]; Immunofluorescence analysis of HeLa cells labelled with ab92454 at 1/500 dilution.