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Home Antibody All anti-NDEL1 antibodies

Anti-NDEL1 TRUEMAB Antibody Clone OTI4E5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503352 NDEL1 (Nudel ) mouse monoclonal antibody, clone OTI4E5 (formerly 4E5) 100ul $325 In Stock
LC422448 NDEL1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503352 Carrier-free (BSA/glycerol-free) NDEL1 mouse monoclonal antibody, clone OTI4E5 (formerly 4E5) 100ug $450 3-4 weeks
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WB(2)
IHC(2)
IF(1)
FC(1)
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Also for NDEL1 (NM_001025579)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NDEL1(NP_001020750) produced in HEK293 cell.
Clone NameClone OTI4E5 IsotypeIgG2a
Species ReactivityHuman Concentration0.57 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 36.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nudE neurodevelopment protein 1-like 1 (NDEL1), transcript variant 1
Alternative NameEOPA; MITAP1; NDE1L1; NDE2; NUDEL
Database LinkNP_001020750
Entrez Gene 81565 Human
FunctionThis gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NDEL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503352)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503352)
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Anti-NDEL1 mouse monoclonal antibody (TA503352) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDEL1(RC212323).
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HEK293T cells transfected with either RC212323 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NDEL1 antibody(TA503352), and then analyzed by flow cytometry.
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