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Anti-NCOA3 Antibody EPR4374(3)
Also for NCOA3 (NM_001174087)
|A synthetic peptide corresponding to residues on the C-terminus in human SRC-3 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:50 - 1:100; FC: 1:100 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens nuclear receptor coactivator 3 (NCOA3), transcript variant 3|
|ACTR; AIB-1; AIB1; bHLHe42; CAGH16; CTG26; KAT13B; pCIP; RAC3; SRC-3; SRC3; TNRC14; TNRC16; TRAM-1|
|SRC-3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. It has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. SRC-3 is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. In addition, a polymorphic repeat region is found in the C-terminus of the protein (1). |
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Western blot - Anti-KAT13B / AIB1 antibody [EPR4374(3)]; All lanes : Anti-SRC3 antibody [EPR4374(3)] at 1/1000 dilution.Lane 1 : Daudi cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 155 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13B / AIB1 antibody [EPR4374(3)]; Immunohistochemical analysis of KAT13B / AIB1 in paraffin embedded Human endometrium adenocarcinoma tissue, using ab133611 at a dilution of 1/50.
Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)]; Overlay histogram showing HeLa cells stained with ab133611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.