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Home Antibody All anti-NCOA3 antibodies

Anti-NCOA3 Antibody EPR4374(3)

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SKU Description Amount Price Availability*  
TA310998
  • Rabbit Monoclonal antibody against SRC-3 (NCOA3)
100ul $325 In Stock
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus in human SRC-3 was used as an immunogen.
Clone NameEPR4374(3) IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:50 - 1:100; FC: 1:100 - 1:500
BufferpH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens nuclear receptor coactivator 3 (NCOA3), transcript variant 3
Alternative NameACTR; AIB-1; AIB1; bHLHe42; CAGH16; CTG26; KAT13B; pCIP; RAC3; SRC-3; SRC3; TNRC14; TNRC16; TRAM-1
Database LinkNP_001167558
Entrez Gene 8202 Human
FunctionSRC-3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. It has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. SRC-3 is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. In addition, a polymorphic repeat region is found in the C-terminus of the protein (1).
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-KAT13B / AIB1 antibody [EPR4374(3)]; All lanes : Anti-SRC3 antibody [EPR4374(3)] at 1/1000 dilution.Lane 1 : Daudi cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : K562 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 155 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT13B / AIB1 antibody [EPR4374(3)]; Immunohistochemical analysis of KAT13B / AIB1 in paraffin embedded Human endometrium adenocarcinoma tissue, using TA310998 at a dilution of 1/50.
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Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)]; Overlay histogram showing HeLa cells stained with TA310998 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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