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Anti-NCF1 PHOSPHO Antibody

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA311761 Rabbit polyclonal p47 phox/NCF1 (Ser359) antibody(Phospho-specific) 100ul $325 3 Weeks
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WB(1)
IHC(1)
IF(1)
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OriGene Data

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 359 (Q-R-SP-K-P).
Clone Name IsotypeIgG
Species ReactivityHuman Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:5000
BufferPhosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note p47 phox (Phospho-Ser359) antibody detects endogenous levels of p47 phox only when phosphorylated at serine 359.

Reference Data

Target NameHomo sapiens neutrophil cytosolic factor 1 (NCF1)
Alternative NameNCF1A; NOXO2; p47phox; SH3PXD1A
Database LinkNP_000256
Entrez Gene 653361 Human
Function
Related Pathway Chemokine signaling pathwayFc gamma R-mediated phagocytosisLeukocyte transendothelial migration

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
Western blot analysis of extracts from HeLa cells treated with nocodazole (1ug/ml, 18hours) and HeLa cells treated with TSA (400nM, 24hours), using p47 phox (Phospho-Ser359) antibody.The lane on the right is treated with the synthesized peptide.
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IHC Image
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using p47 phox (Phospho-Ser359) antibody.The picture on the right is treated with the synthesized peptide.
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IF Image
Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) antibody.The picture on the right is treated with the synthesized peptide.
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