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Anti-NCF1 PHOSPHO Antibody
Also for NCF1 (NM_000265)
|The antiserum was produced against synthesized phosphopeptide derived from human p47 phox around the phosphorylation site of serine 359 (Q-R-SP-K-P).|
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|p47 phox (Phospho-Ser359) antibody detects endogenous levels of p47 phox only when phosphorylated at serine 359.
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Western blot analysis of extracts from HeLa cells treated with nocodazole (1ug/ml, 18hours) and HeLa cells treated with TSA (400nM, 24hours), using p47 phox (Phospho-Ser359) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using p47 phox (Phospho-Ser359) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) antibody.The picture on the right is treated with the synthesized peptide.