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Anti-NCAM1 Antibody EPR2566
Also for NCAM1 (NM_181351)
|A synthetic peptide corresponding to residues in extracellular domain of human CD56/NCAM was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 2|
|CD56; MSK39; NCAM|
|The neural cell adhesion molecule (NCAM/CD56) is a homophilic binding glycoprotein belonging to immunoglobulin (Ig) superfamily. It is commonly found on the surface of neurons, NK cells, gilas, and neuroendocrine tissues, and it is involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM mediates axon growth, fasciculation, and cell adhesion by homophilic and heterophilic interactions (1). Due to alternative splicing, at least 27 different isoforms of NCAM mRNAs has been identified, leading to variety of NCAM isoforms (2). Three major NCAM isoforms are: 140 and 180 kDa transmembrane forms (NCAM-140, NCAM-180), and 120 kDa glycophosphatidylinositol (GPI)-linked isoform (NCAM120) (3).|
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Western blot - Anti-NCAM antibody [EPR2566]; All lanes : Anti-NCAM antibody [EPR2566] at 1/1000 dilution.Lane 1 : SH-SY5Y cell lysate.Lane 2 : Human fetal brain cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 95 kDa.Observed band size : 140-180 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM antibody [EPR2566]; Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue staining NCAM with ab133345 at 1/100 dilution.