Home Antibody All anti-NANOG antibodies
Also for NANOG (NM_024865)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human NANOG.|
||WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens Nanog homeobox (NANOG)|
|homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox|
|NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.|
Wnt Signaling Pathway
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(LEFT)Western blot analysis of NANOG Antibody (Center) in Hela cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab (1:60 dilution). (RIGHT)Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human testis tissue reacted with NANOG antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.