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Home Antibody All anti-NANOG antibodies

Anti-NANOG Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302153
  • Rabbit Polyclonal Antibody against NANOG (N-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC411030) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)
IHC(1)
IF(2)
FC(1)
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Also for NANOG (NM_024865)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human NANOG.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman (Predicted: Monkey) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IF: 1:10~50, IHC: 1:10~50, FC: 1:10~50
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens Nanog homeobox (NANOG), transcript variant 1
Alternative Namehomeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Database LinkNP_079141
Entrez Gene 79923 Human
Entrez Gene 715746 Monkey
FunctionNANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Related PathwayES Cell Differentiation/IPSStem cell - PluripotencyCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cells

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#TA302153) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#TA302153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term)(Cat.#TA302153), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF Image
Fluorescent confocal image of SY5Y cells stained with TA302153 NANOG (N-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with TA302153 NANOG (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.
IF Image
Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term)(Cat#TA302153).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37â). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37â).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37â). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.
FC Image
NANOG Antibody (N-term) (Cat. #TA302153) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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