* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for NANOG (NM_024865)
OriGene Data
| This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NANOG. |
|
Rabbit Ig |
| Human |
0.25 mg/ml |
| WB |
WB: 1:50~100; ELISA: 1: 1,000; |
| Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. (Protein A or G Sepharose) |
Reference Data
| Homo sapiens Nanog homeobox (NANOG) |
| homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox |
| NP_079141 |
| NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. |
Wnt Signaling Pathway |
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WB Image
Western blot analysis of anti-NANOG Antibody (N-term) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab (1:60 dilution). Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
