Home Antibody All anti-NANOG antibodies
Also for NANOG (NM_024865)
|This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human NANOG.|
|Human (Predicted: Monkey)
|WB, IHC, IF, FC
||WB: 1:1000, IF: 1:10~50, IHC: 1:10~50, FC: 1:10~50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|Homo sapiens Nanog homeobox (NANOG), transcript variant 1|
|homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox|
Entrez Gene 79923 Human
Entrez Gene 715746 Monkey
|NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.|
|ES Cell Differentiation/IPSStem cell - PluripotencyCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cells |
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Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#TA302153) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#TA302153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term)(Cat.#TA302153), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of SY5Y cells stained with TA302153 NANOG (N-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with TA302153 NANOG (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (5.25 Î¼M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 Âµg/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.
Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term)(Cat#TA302153).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37Ã¢). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37Ã¢).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37Ã¢). Nuclei were counterstained with DAPI (blue) (10 Âµg/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.
NANOG Antibody (N-term) (Cat. #TA302153) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.