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Home Antibody All anti-NAMPT antibodies

Anti-NAMPT Antibody EPR3392

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307197
  • Rabbit monoclonal antibody against PBEF(clone EPR3392)
100ul $325 Next Day
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WB(2)
IHC(7)
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Also for NAMPT (NM_005746)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PBEF was used as an immunogen.
Clone NameEPR3392 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: 1:250 - 1:500; ICC: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens nicotinamide phosphoribosyltransferase (NAMPT)
Alternative Name1110035O14Rik; PBEF; PBEF1; VF; VISFATIN
Database LinkNP_005737
FunctionPBEF (nicotinamide phosphoribosyltransferase) catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. This protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. PBEF is highly expressed in visceral fat, so serum levels of the protein correlate with obesity.
Related Pathway
Adipogenesis

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WB Image
Western blot - PBEF antibody [EPR3392]; All lanes : Anti-Visfatin antibody [EPR3392] at 1/1000 dilution.Lane 1 : Fetal liver lysate.Lane 2 : Human skeletal muscle lysate.Lane 3 : Fetal kidney lysate.Lane 4 : Human heart lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 56 kDa.Observed band size : 52 kDa .
WB Image
Western blot - Anti-PBEF antibody [EPR3392]; All lanes : Anti-Visfatin antibody [EPR3392] at 1/1000 dilution.Lane 1 : Mouse brain lysate.Lane 2 : Mouse heart lysate.Lane 3 : Mouse kidney lysate.Lane 4 : Mouse spleen lysate.Lane 5 : Rat brain lysate.Lane 6 : Rat heart lysate.Lane 7 : Rat kidney lysate.Lane 8 : Rat spleen lysate.Predicted band size : 56 kDa.Observed band size : 56 kDa.Additional bands at : 120 kDa,45 kDa. We are unsure as to the identity of these extra bands.Exposure time : 3 minutes
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392]; TA307197, at 1/250 dilution, staining PBEF in paraffin-embedded Human kidney tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392]; TA307197, at 1/250 dilution, staining PBEF in paraffin-embedded Human liver tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBEF antibody [EPR3392]; TA307197 staining at 1/250 dilution staining PBEF in mouse liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PBEF antibody [EPR3392](TA307197); TA307197 showing positive staining in Normal placenta tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PBEF antibody [EPR3392](TA307197); TA307197 showing negative staining in Normal tonsil tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PBEF antibody [EPR3392](TA307197); TA307197 showing negative staining in Normal uterus tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PBEF antibody [EPR3392](TA307197); TA307197 showing positive staining in Skeletal muscle tissue.

 

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