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Home Antibody All anti-MYL2 antibodies

Anti-MYL2 Antibody EPR3741

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307708
  • Rabbit monoclonal antibody against Myosin Light Chain 2 (clone EPR3741 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400153) , 20ug Explanation
100ul 325 In Stock
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WB(2)
IHC(8)
Assay(1)
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Also for MYL2 (NM_000432)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Myosin Light Chain 2 was used as an immunogen.
Clone NameEPR3741 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsIHC: 1:100 - 250, IP: 1:50, WB: 1:10,000 - 50,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens myosin, light chain 2, regulatory, cardiac, slow (MYL2)
Alternative NameCMH10; MLC2
Database LinkNP_000423
Entrez Gene 4633 Human
Entrez Gene 17906 Mouse
Entrez Gene 363925 Rat
FunctionMyosin is a hexamer of 2 identical heavy chains and 2 pairs of light chains (1). The two pairs of light chains of muscle myosins are called essential light chains (ELC) and regulatory light chains (RLC). The light chains stabilize the long alpha helical neck of the myosin head (2). Myosin light chain-2 (MYL2), also known as the regulatory light chain of myosin, is an important protein involved in the regulation of myosin ATPase activity in smooth muscle (3). Calcium triggers the phosphorylation of MYL2 that in turn triggers contraction. The phosphorylation sites of MYL2 are mainly Serine 19 (monophosphorylation) and, in some conditions, both Threonine 18 and Serine 19 (diphosphorylation) (4). An increase in ventricular MYL2 is observed during myocardial hypertrophy in cardiac patients with valve stenosis (3).
Related Pathway Cardiac muscle contractionFocal adhesionTight junctionLeukocyte transendothelial migrationRegulation of actin cytoskeletonHypertrophic cardiomyopathy (HCM)More Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Myosin Light Chain 2 antibody [EPR3741]; All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] at 1/10000 dilution.Lane 1 : Fetal heart lysate.Lane 2 : Fetal skeletal muscle lysate.Lane 3 : Human heart lysate.Lane 4 : Mouse heart lysate.Lane 5 : Rat heart lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 19 kDa.
WB Image
Western blot ; All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] at 1/10000 dilution.Lane 1 : Human Myosin Light Chain 2 full length protein at 0.1 ug.Lane 2 : Human Myosin Light Chain 2 full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 4 minutes
Assay Image
Other-Anti-Myosin Light Chain 2 antibody [EPR3741](TA307708); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin Light Chain 2 antibody [EPR3741]; TA307708, at a 1/100 dilution, staining Myosin Light Chain 2 in paraffin embedded Human heart muscle by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myosin Light Chain 2 antibody [EPR3741](TA307708); TA307708 showing positive staining in Heart muscle tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myosin Light Chain 2 antibody [EPR3741](TA307708); TA307708 showing positive staining in Skeletal muscle tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myosin Light Chain 2 antibody [EPR3741](TA307708); TA307708 showing negative staining in Normal tonsil tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myosin Light Chain 2 antibody [EPR3741](TA307708); TA307708 showing negative staining in Normal brain tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myosin Light Chain 2 antibody [EPR3741](TA307708); TA307708 showing negative staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myosin Light Chain 2 antibody [EPR3741](TA307708); TA307708 showing negative staining in Normal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody [EPR3741]; TA307708 showing negative staining in Normal breast tissue.

 

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