OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-MYC antibodies

Anti-MYC PHOSPHO Antibody E203

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303607
  • Rabbit monoclonal antibody against Phospho-c-Myc (pT58/S62)(E203) (phospho-specific)
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IF(1)
FC(1)
spacer
Also for MYC (NM_002467)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequ
Clone NameE203 IsotypeRabbit IgG
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsWB: 1:1,000; ICC: 1:50-100; IP: 1:100
Buffer50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Reference Data

Target NameHomo sapiens v-myc avian myelocytomatosis viral oncogene homolog (MYC)
Alternative NamebHLHe39; c-Myc; MRTL; MYCC
Database LinkNP_002458
FunctionThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq].
Related Pathway
Apoptosis
Cell cycle
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - c-Myc (phospho T58 + S62) antibody [E203]; All lanes : Anti-c-Myc (phospho T58 + S62) antibody [E203] at 1/1000 dilution.Lane 1 : Untreated A431 cell lysate.Lane 2 : TPA treated A431 cell lysate.Predicted band size : 49 kDa.Observed band size : 57 kDa .
IF Image
Immunofluorescence - c-Myc (phospho T58 + S62) antibody [E203]; Immunofluorescent staining of A431 cells
FC Image
Flow Cytometry - Anti-c-Myc (phospho T58 + S62) antibody [E203]; Overlay histogram showing Jurkat cells stained with TA303607 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title