OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
Home Antibody All anti-MTOR antibodies

Anti-MTOR PHOSPHO Antibody EPR426(2)


Specifications Citations (1) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA307262 Rabbit monoclonal antibody against mTOR/FRAP Phospho (pS2448)(clone EPR426(2)) (Phospho-Specific) 100ul $325 In Stock
Add to Shopping Cart
Write Review & Get Rewarded

Write a review and get rewarded!

Review this antibody and earn an OriGene coupon or Amazon giftcard

Follow these easy steps to reward yourself:

  1. Sign in to your account, or register for a new account;
  2. Write a review;
  3. Receive a coupon or giftcard once your review is accepted.

Click the button to start writing a review

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding serine 2448 of human mTOR/FRAP was used as an immunogen. This antibody only detects mTOR/FRAP phosphorylated at serine 2448.
Clone NameEPR426(2) IsotypeIgG
Species ReactivityMouse, Rat, Human, Pig ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250; FC: 1:100
Predicted MW Explanation 289 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR)
Alternative NameFRAP; FRAP1; FRAP2; RAFT1; RAPT1
Database LinkNP_004949
Entrez Gene 2475 Human
Entrez Gene 56717 Mouse
Entrez Gene 56718 Rat
FunctionThe mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-terminal half of 20 tandem HEAT repeats that are implicated in protein-protein interactions and a C-terminal half that includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity, and the deletion of a single amino acid from this domain abrogates this activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the mRNA translation repressor, 4E-BP1, and also through the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Moreover, other known mediators of mTOR include PI3-K and ATK from the insulin pathway.
Related PathwayProtein KinaseDruggable Genome ErbB signaling pathwaymTOR signaling pathwayInsulin signaling pathwayAdipocytokine signaling pathwayType II diabetes mellitusPathways in cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - mTOR (phospho S2448) antibody [EPR426(2)]; All lanes : Anti-mTOR (phospho S2448) [EPR426(2)] antibody at 1/1000 dilution.Lane 1 : 293T cell lysate, untreated.Lane 2 : 293T cell lysate, treated with Alkaline Phosphatase .Lysates/proteins at 10 ug per lane.Predicted band size : 289 kDa.

WB Image
Figure from citation: Western blot analysis of phosphorylated-mTOR (pmTOR), mTOR, LC3-I and LC3-II protein levels by using anti-MTOR phospho, anti-MTOR and anti-MAP1LC3B antibody in wild-type and wood dust-initiated Beas-2B cells. View Citation

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mTOR (phospho S2448) antibody [EPR426(2)]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using TA307262 at a dilution of 1/50.

IF Image
Immunocytochemistry/ Immunofluorescence - mTOR (phospho S2448) antibody [EPR426(2)]; Immunofluorescent staining of HeLa cells using TA307262 at a dilution of 1/100.

FC Image
Flow Cytometry - Anti-mTOR (phospho S2448) antibody; Overlay histogram showing HeLa cells stained with TA307262 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HeLa cells under the same conditions.


Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.