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Anti-MTOR PHOSPHO Antibody EPR426(2)
Also for MTOR (NM_004958)
|A phospho specific peptide corresponding to residues surrounding serine 2448 of human mTOR/FRAP was used as an immunogen. This antibody only detects mTOR/FRAP phosphorylated at serine 2448.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR)|
|FRAP; FRAP1; FRAP2; RAFT1; RAPT1|
|The mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-terminal half of 20 tandem HEAT repeats that are implicated in protein-protein interactions and a C-terminal half that includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity, and the deletion of a single amino acid from this domain abrogates this activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the mRNA translation repressor, 4E-BP1, and also through the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Moreover, other known mediators of mTOR include PI3-K and ATK from the insulin pathway.|
Senescence and Autophagy
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Western blot - mTOR (phospho S2448) antibody [EPR426(2)]; All lanes : Anti-mTOR (phospho S2448) [EPR426(2)] antibody at 1/1000 dilution.Lane 1 : 293T cell lysate, untreated.Lane 2 : 293T cell lysate, treated with Alkaline Phosphatase .Lysates/proteins at 10 µg per lane.Predicted band size : 289 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mTOR (phospho S2448) antibody [EPR426(2)]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109268 at a dilution of 1/50.
Immunocytochemistry/ Immunofluorescence - mTOR (phospho S2448) antibody [EPR426(2)]; Immunofluorescent staining of HeLa cells using ab109268 at a dilution of 1/100.
Flow Cytometry - Anti-mTOR (phospho S2448) antibody [EPR426(2)]; Overlay histogram showing HeLa cells stained with ab109268 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.