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Anti-MTOR PHOSPHO Antibody EPR426(2)
Also for MTOR (NM_004958)
|A phospho specific peptide corresponding to residues surrounding serine 2448 of human mTOR/FRAP was used as an immunogen. This antibody only detects mTOR/FRAP phosphorylated at serine 2448.|
|Mouse, Rat, Human, Pig
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IP.
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Western blot - mTOR (phospho S2448) antibody [EPR426(2)]; All lanes : Anti-mTOR (phospho S2448) [EPR426(2)] antibody at 1/1000 dilution.Lane 1 : 293T cell lysate, untreated.Lane 2 : 293T cell lysate, treated with Alkaline Phosphatase .Lysates/proteins at 10 ug per lane.Predicted band size : 289 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mTOR (phospho S2448) antibody [EPR426(2)]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using TA307262 at a dilution of 1/50.
Immunocytochemistry/ Immunofluorescence - mTOR (phospho S2448) antibody [EPR426(2)]; Immunofluorescent staining of HeLa cells using TA307262 at a dilution of 1/100.
Flow Cytometry - Anti-mTOR (phospho S2448) antibody [EPR426(2)]; Overlay histogram showing HeLa cells stained with TA307262 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.