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Anti-MST4 PHOSPHO Antibody EP2123Y
Also for MST4 (NM_016542)
|A phospho specific peptide corresponding to residues surrounding threonine 178 of human MST4, theonine 190 of human MSt 3 and threonine 174 of human STK25.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000; ICC: 1:100 - 250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens serine/threonine protein kinase MST4 (MST4), transcript variant 1|
|The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]. |
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Western blot - MST4 + MST3 + STK25 (phospho T178 + T190 + T174) antibody [EP2123Y]; All lanes : Anti-MST4 + MST3 + STK25 (phospho T178 + T190 + T174) antibody [EP2123Y] at 1/2000 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with calyculin A.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 47-49 kDa.Observed band size : 52 kDa .