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Anti-MSH2 Antibody EPR3943
Also for MSH2 (NM_000251)
|A synthetic peptide corresponding to residues on the N-terminus in human MSH2 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for ICC/IF or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC/IF or IP.
|Homo sapiens mutS homolog 2 (MSH2), transcript variant 1|
|COCA1; FCC1; HNPCC; HNPCC1; LCFS2|
|MutS homologue 2 (MSH2) is a DNA mismatch repair protein in the MutS family (1). MSH2 forms two different heterodimers: MutS alpha (MSH2-MSH6) and MutS beta (MSH2-MSH3) that bind to DNA mismatches thereby initiating DNA repair. (2). MutS alpha bends the DNA helix and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA (2). MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long (2). Heterozygous mutations in the MSH2 gene are a cause of hereditary nonpolyposis colorectal cancer (HNPCC), forming a specific mispair binding complex with MSH3 and MSH6 (3, 4). MutS alpha may also play a role in DNA homologous recombination repair (2).|
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Western blot - MSH2 antibody [EPR3943]; All lanes : Anti-MSH2 antibody [EPR3943] at 1/1000 dilution.Lane 1 : A375 cell lysate.Lane 2 : A431 cell lysate.Lane 3 : SW480 cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 105 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MSH2 antibody [EPR3943]; ab92473, at a 1/100 dilution, staining MSH2 in paraffin embedded Human colonic adenocarcinoma by Immunohistochemistry. Detection utilised HRP conjugated anti-rabbit antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [EPR3943]; ab92473 (1/500) staining MSH2 in assynchronous Hela cells (green). Cells were fixed in formaldehyde, permeabilised with 0.5% Triton X-100 (in PBS) and counterstained with DAPI in order to highlight the nuceleus (red). For further experimental details please refer to Abreview.
Immunocytochemistry/ Immunofluorescence - MSH2 antibody [EPR3943]; ab92473, at a 1/100 dilution, staining MSH2 in HeLa cells by Immunofluorescence.