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Home Antibody All anti-MRPS27 antibodies

Anti-MRPS27 TRUEMAB Antibody Clone OTI1A5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA800223
  • MRPS27 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402416) , 20ug Explanation
100ul $325 In Stock
CF800223
  • Carrier-free (BSA/glycerol-free) MRPS27 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)
100ug $450 8-10 Days

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(2)
FC(1)
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Also for MRPS27 (NM_015084)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 151-414 of human MRPS27 (NP_055899) produced in E.coli.
Clone NameClone OTI1A5 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:200, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 47.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitochondrial ribosomal protein S27 (MRPS27), transcript variant 2
Alternative NameMRP-S27; S27mt
Database LinkNP_055899
Entrez Gene 23107 Human
FunctionMammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (RC209478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800223)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MRPS27 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800223)
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HEK293T cells transfected with either RC209478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MRPS27 antibody(TA800223), and then analyzed by flow cytometry.
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