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Also for MRE11 (NM_005590)
|This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 578-590 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein.|
||ELISA: 1:10,000 - 1:50,000, WB: 1:500- 1:2,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Mre11 (also known as double-strand break repair protein MRE11) is a subunit of a complex with Rad50 and Xrs2 (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability. Mre11 possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity that appears to be required for RMX function. This nuclear protein is widely conserved and is also involved in meiotic double strand break processing.
|Homo sapiens MRE11 meiotic recombination 11 homolog A (S. cerevisiae) (MRE11A), transcript variant 2|
|ATLD; HNGS1; MRE11; MRE11B|
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Chromatin Immunoprecipitation (ChIP) using Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green® (Invitrogen), and detected using a Fuji scanning fluorometer. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.