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Home Antibody All anti-MMP9 antibodies

Anti-MMP9 Antibody EP1254

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303603
  • Rabbit monoclonal antibody against MMP-9 (EP1254 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401553) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(2)
FC(1)
Assay(1)
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Also for MMP9 (NM_004994)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on human MMP-9 was used as an immunogen.
Clone NameEP1254 IsotypeTissue culture supernatant
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsICC/IF: 1:250 - 1:500; WB: 1:5000 - 1:20000; IHC-P: 1:100 - 1:250; FC: 1:1000
BufferDoes not react with Mouse. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9)
Alternative NameCLG4B; GELB; MANDP2; MMP-9
Database LinkNP_004985
Entrez Gene 4318 Human
Entrez Gene 81687 Rat
FunctionProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq].
Related PathwayProteaseDruggable Genome Leukocyte transendothelial migrationPathways in cancerBladder cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MMP9 antibody [EP1254]; All lanes : Anti-MMP9 antibody [EP1254] at 1/10000 dilution.Lane 1 : U937 cell lysate treated with TPA.Lane 2 : HL60 cell lysate treated with TPA.Lane 3 : HT1080 cell lysate treated with TPA.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 78 kDa.Observed band size : 92 kDa .
Assay Image
Other-Anti-MMP9 antibody [EP1254](TA303603); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP9 antibody [EP1254]; TA303603 at 1/100-1/250 dilution staining MMP9 in human gastic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MMP9 antibody [EP1254]; TA303603 staining MMP9 in U87-MG cells treated with domoic acid , by ICC/IF. Increase of MMP9 expression correlates with increased concentration of domoic acid, as described in literature.The cells were incubated at 37°C for 6h in media containing different concentrations of (domoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA303603 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
IF Image
Immunocytochemistry/ Immunofluorescence - MMP9 antibody [EP1254]; TA303603 at 1/250-1/500 dilution staining MMP9 in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - MMP9 antibody [EP1254]; Overlay histogram showing permeabilized A431 cells stained with TA303603 (pink line). Negative control antibody (green line) was rabbit IgG.

 

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