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Anti-MLH1 Antibody EPR3893
Also for MLH1 (NM_000249)
|A synthetic peptide corresponding to residues in human MLH1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens mutL homolog 1 (MLH1), transcript variant 1|
|COCA2; FCC2; hMLH1; HNPCC; HNPCC2|
Entrez Gene 4292 Human
Entrez Gene 17350 Mouse
Entrez Gene 81685 Rat
|MLH1 is a DNA mismatch repair protein that heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, and then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in the presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the mismatch strand. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may recruit DNA polymerase III to the site of the MMR. Defects in MLH1 are the cause of hereditary nonpolyposis colorectal cancer type 2 (HNPCC2). Most patients with HNPCC have mutations in either the MLH1 or MSH2 genes (1).|
|Druggable Genome Mismatch repairPathways in cancerColorectal cancerEndometrial cancer|
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Western blot - MLH1 antibody [EPR3893]; All lanes : Anti-MLH1 antibody [EPR3893] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : K562 cell lysate.Lane 4 : SH-SY5Y cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 85 kDa.