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Home Antibody All anti-MLH1 antibodies

Anti-MLH1 Antibody EPR3894

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307733
  • Rabbit monoclonal antibody against MLH1(clone EPR3894)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400096) , 20ug
100ul 325 In Stock
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WB(1)
IHC(3)
IF(1)
FC(1)
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Also for MLH1 (NM_000249)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human MLH1 was used as an immunogen.
Clone NameEPR3894 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100; ICC/IF: 1:200
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens mutL homolog 1 (MLH1), transcript variant 1
Alternative NameCOCA2; FCC2; hMLH1; HNPCC; HNPCC2
Database LinkNP_000240
FunctionMLH1 is a DNA mismatch repair protein that heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, and then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in the presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the mismatch strand. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may recruit DNA polymerase III to the site of the MMR. Defects in MLH1 are the cause of hereditary nonpolyposis colorectal cancer type 2 (HNPCC2). Most patients with HNPCC have mutations in either the MLH1 or MSH2 genes (1).
Related Pathway

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WB Image
Western blot - MLH1 antibody [EPR3894]; All lanes : Anti-MLH1 antibody [EPR3894] at 1/10000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lane 4 : SW480 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 84 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLH1 antibody [EPR3894]; TA307733 at 1/100 dilution staining MLH1 in Human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894]; TA307733 staining MLH1 in Human colorectal (top) and gastric tissue (bottom) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLH1 antibody [EPR3894]; TA307733 at 1/100 dilution staining MLH1 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894]; TA307733 (1/200) staining MLH1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.
FC Image
Flow Cytometry-Anti-MLH1 antibody [EPR3894](TA307733); Overlay histogram showing HeLa cells stained with TA307733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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