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Home Antibody UltraMAB

UltraMAB

KI67 UltraMAB Antibody, Clone UMAB107

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (1) Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM800033
  • Purified KI67 (MKI67) mouse monoclonal antibody,clone UMAB107, 100 ul
$468 In Stock Add to Shopping Cart
UM870033
  • MKI67 mouse monoclonal antibody,clone UMAB107, 30 ul
$120 In Stock Add to Shopping Cart
WB(2)
Data Img
IHC(5)
Data Img
IF(1)
Data Img

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli.
Clone NameClone UMAB107 IsotypeIgG2a
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC: 1:300
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens marker of proliferation Ki-67 (MKI67), transcript variant 1
Alternative NameKIA; MIB-; MIB-1; PPP1R105
Database LinkNP_002408
FunctionThis gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western Blot analysis of HT-29 and NCI-H322M cell lysates (35µg) by using anti-KI67 monoclonal antibody (Clone UMAB107)
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Western Blot analysis of MOLT-4 and MDA-MB231 cell lysates (35µg) by using anti-KI67 monoclonal antibody (Clone UMAB107)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human colon cancer tissue using anti-MKI67 mouse monoclonal antibody. (UM800033 at 1ug/ml for 30 min at RT; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 20min; )
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Immunohistochemical staining of paraffin-embedded normal human tonsil tissue using anti-MKI67 mouse monoclonal antibody. (UM800033 at 1ug/ml for 30 min at RT; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 20min; )
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Immunohistochemical staining of paraffin-embedded mouse colon tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120C for 3min)
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Immunofluorescent staining of MDA-MB-231 cells using anti-KI67 mouse monoclonal antibody (UM800033, green, 1:100). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
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