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Home Antibody All anti-METAP2 antibodies

Anti-METAP2 Antibody EPR6886(B)


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SKU Description Amount Price Availability*  
TA310688 Rabbit Monoclonal antibody against METAP2 100ul $325 In Stock
LC402045 METAP2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart


OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human METAP2 was used as an immunogen.
Clone NameEPR6886(B) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; FC: 1:10 - 1:100; IHC-Fr: Use at an assay dependent concentration
Predicted MW Explanation 52 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC/IF,IHC-P or IP.

Reference Data

Target NameHomo sapiens methionyl aminopeptidase 2 (METAP2)
Alternative NameMAP2; MNPEP; p67; p67eIF2
Database LinkNP_006829
Entrez Gene 10988 Human
Entrez Gene 56307 Mouse
Entrez Gene 64370 Rat
FunctionMETAP2 is a member of the methionyl aminopeptidase family and binds 2 cobalt or manganese ions. It functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of METAP2 is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site (1).
Related PathwayProteaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)]; All lanes : Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] at 1/1000 dilution.Lane 1 : HepG2 cell lysates.Lane 2 : 293T cell lysates.Lane 3 : HeLa cell lysates.Lane 4 : C6 cell lysates.Lane 5 : RAW 264.7 cell lysates.Lane 6 : PC12 cell lysates.Lane 7 : NIH 3T3 cell lysates.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 67 kDa .
Immunohistochemistry (Frozen sections) - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)]; TA310688 staining Methionine Aminopeptidase 2 in Mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with Acetone and blocked with 5% Serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/25 in PBS + 5% goat serum) for 1 hours at 25°C. An Alexa Fluor?488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
Flow Cytometry - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)]; TA310688 at 1/10 dilution staining Methionine Aminopeptidase 2 in permeabilized 293T cells by Flow cytometry (red) or a Rabbit IgG (negative control) (green).


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