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Home Antibody All anti-MET antibodies

Anti-MET Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319135
  • Rabbit polyclonal c-Met phospho Y1349 / Y1356 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400094) , 20ug Explanation
100ug 325 3-7 Days
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WB(2)
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Also for MET (NM_000245)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsELISA: 1:93,000, WB: 1ug/mL
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is designed, produced, and validated for Cancer, Immunology and Nuclear Signaling research. Anti-c-MET is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines.  Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation.  When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others.  C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission.  Well-characterized downstream signalling pathways that are activated by c-Met include the ERK/MAPK, PI3K–Akt/PKB, Crk–Rap and Rac–Pak pathways, resulting in proliferation and increased cell survival.

Reference Data

Target NameHomo sapiens MET proto-oncogene, receptor tyrosine kinase (MET), transcript variant 2
Alternative NameAUTS9; c-Met; HGFR; RCCP2
Database LinkNP_000236
Entrez Gene 4233 Human
Entrez Gene 17295 Mouse
Entrez Gene 24553 Rat
Function
Related PathwayProtein KinaseTransmembraneDruggable Genome Cytokine-cytokine receptor interactionEndocytosisAxon guidanceFocal adhesionAdherens junctionEpithelial cell signaling in Helicobacter pylori infectionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF. Cell lysates were immunoprecipated with the anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-c-Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD
WB Image
Western blot using Rockland’s affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were immunoprecipitated using a human anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356 antibody (#TA319135), lane 3 and 4 where probed using an anti-phosphotyrosine antibody as phosphorylation control and lane 5 and 6 where probed using an anti-cMet antibody as a total Met loading control. Bands recognized at an apparent molecular weight of ~170 kDa are total Met, ~145 kDa phosphorylated Met beta chain in HGF +, ~150 kDa phosphorylated Met in +/- HGF, ~50kDa phosphorylated Met alpha chain. Personal communication with D. Bottaro and F. Cecchi, CCR-NCI, Bethesda, MD.

 

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