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Anti-MET PHOSPHO Antibody EP2367Y
|A phospho specific peptide corresponding to residues surrounding tyrosine 1349 of human Met was used as an immunogen. This antibody detects Met phosphorylated at tyrosine 1349.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000; IHC: 1:50; ICC: 1:50; IP: 1:20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens met proto-oncogene (MET), transcript variant 2|
|AUTS9; c-Met; HGFR; RCCP2|
|Met is a receptor protein-tyrosine kinase (RPTK) for hepatocyte growth factor (HGF), which is a multifunctional cytokine controlling cell growth, morphogenesis, and motility. Met overexpression has been identified in a variety of human cancers (1). Met kinase domain possesses unique features that distinguish met from other members of the src family of protein tyrosine kinases. These results also demonstrate that the product of the activated met gene is a fusion protein and that the amino terminal end of this fusion protein exhibits homology to laminin B1 (2). Data suggest that RanBP9, functioning as an adaptor protein for the Met tyrosine kinase domain, can augment the HGF-Met signaling pathway and that RanBP9 overexpression may cause constitutive activation of the Ras signaling pathway (2). Interaction of the hepatocyte growth factor (HGF) with its receptor, the Met tyrosine kinase, results in invasive growth, a genetic program essential to embryonic development and implicated in tumor metastasis. Met-mediated invasive growth requires autophosphorylation of the receptor on tyrosines located in the kinase activation loop (Tyr(1234)-Tyr(1235)) and in the tail (Tyr(1349)-Tyr(1356)). |
Cytokine-cytokine receptor interaction
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Western blot - Met (c-Met) (phospho Y1349) antibody [EP2367Y]; All lanes : Anti-Met (c-Met) (phospho Y1349) antibody [EP2367Y] at 1/10000 dilution.Lane 1 : A431 cell lysate, untreated.Lane 2 : A431 cell lysate, treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 156 kDa.Observed band size : ~150 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Met (c-Met) (phospho Y1349) antibody [EP2367Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68141 at a dilution of 1/50.