Home Antibody All anti-MEF2C antibodies
Also for MEF2C (NM_002397)
|This MEF2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-394 amino acids from human MEF2C.|
|Human (Predicted: Pig)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000, IF: 1:10~50, IHC: 1:10~50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is purified through a protein A column, followed by peptide affinity purification.
|Homo sapiens myocyte enhancer factor 2C (MEF2C), transcript variant 1|
Entrez Gene 4208 Human
|MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.|
|Transcription Factors MAPK signaling pathway|
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MEF2C Antibody (pS387) (Cat. #TA302220) western blot analysis in HL-60,K562 cell line lysates (35ug/lane).This demonstrates the MEF2C antibody detected the MEF2C protein (arrow).
Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387) (Cat.#TA302220), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387)(Cat#TA302220).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25, 1 h at 37Ã¢). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37Ã¢).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37Ã¢). Nuclei were counterstained with DAPI (blue) (10 Âµg/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.