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Home Antibody All anti-MDM2 antibodies
UltraMAB

Anti-MDM2 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590122
  • Rabbit Polyclonal MDM2 Antibody
50ug 325 In Stock
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(11)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens MDM2 proto-oncogene, E3 ubiquitin protein ligase (MDM2), transcript variant 1
Alternative NameACTFS; hdm2; HDMX
Database LinkNP_002383
Entrez Gene 4193 Human
FunctionMDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53 mediated transactivation, MDM2 also binds to p53 protein. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2 p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full length MDM2 transcript is expressed in normal tissue.
Related PathwayTranscription FactorsDruggable Genome Cell cyclep53 signaling pathwayUbiquitin mediated proteolysisEndocytosisPathways in cancerGliomaMore Pathways >>

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM2 (RC219518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM2.
ELISA Image
ELISA: MDM2 Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MDM2 Rabbit Polyclonal antibody. (TA590122)

 

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