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Home Antibody All anti-MCM7 antibodies

Anti-MCM7 Antibody

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SKU Description Amount Price Availability*  
TA308526 Rabbit polyclonal antibody to MCM7 (minichromosome maintenance complex component 7) 100ul $325 In Stock

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WB(1)
IP(1)
IHC(1)
IF(1)
Assay(1)

OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 190 and 481 of MCM7 (Uniprot ID#P33993)
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Rat, Zebrafish, Bovine) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC, IF, IP Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 61kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Mouse (92%), Rat (93%), Zebrafish (85%), Bovine (98%)

Reference Data

Target NameHomo sapiens minichromosome maintenance complex component 7 (MCM7), transcript variant 2
Alternative NameCDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
Database LinkNP_877577
Entrez Gene 4176 Human
Entrez Gene 17220 Mouse
Entrez Gene 288532 Rat
FunctionThe protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
Related PathwayTranscription Factors DNA replicationCell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Sample (30 ug of whole cell lysate). A: H1299. 7.5% SDS PAGE. TA308526 diluted at 1:1000.
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Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
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Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using MCM7(TA308526) antibody at 1:100 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM7(TA308526) antibody at 1:200 dilution.
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MCM7 antibody [N2C2], Internal immunoprecipitates MCM7 protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 ug HeLa whole cell extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of MCM7 protein by 4 ug MCM7 anti
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