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Home Antibody All anti-MCM5 antibodies

Anti-MCM5 Antibody EP2683Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301223
  • Rabbit Monoclonal Antibody against MCM5 (Clone EP2683Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402012) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(8)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of human MCM5 was used as an immunogen.
Clone NameEP2683Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:50; IHC-P: 1:250 - 1:500; FC: 1:40; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 82 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens minichromosome maintenance complex component 5 (MCM5)
Alternative NameCDC46; P1-CDC46
Database LinkNP_006730
Entrez Gene 4174 Human
Entrez Gene 17218 Mouse
FunctionHuman nuclear proteins MCM3 and MCM5 have high sequence similarities with the corresponding yeast proteins known to be required for the initiation of genome replication. At 0.5 M NaCl, the structure-bound nuclear protein can be partially solubilized as a dimer composed of MCM3 and the related protein MCM5. MCM3 forms stable complexes with protein MCM5. These MCM3/MCM5 complexes occur as dimers and in high-molecular-mass complexes (approximately 500 kDa). The high-molecular-mass complexes dissociate in 0.5 M NaCl and release MCM3/MCM5 dimers. It has frequently been proposed that the MCM proteins may function as licensing factors for genome replication. Data imply that the active form of an MCM protein is not a monomer, but a protein complex that includes an MCM3/MCM5 dimer (1). Levels of member of the MCM family (MCM2, MCM3, MCM5 and MCM7) gradually increased in a variable manner as KD cells progressed from GO into the G1/S phase. In the GO stage, the amounts of MCM2 and -5 proteins were much lower than those of MCM7 and -3 proteins, suggesting that they are not present in stoichiometric amounts, and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM/P1 complexes (2).
Related PathwayStem cell - PluripotencyTranscription Factors DNA replicationCell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MCM5 antibody [EP2683Y]; All lanes : Anti-MCM5 antibody [EP2683Y] at 1/10000 dilution.Lane 1 : HeLa lysate.Lane 2 : MCF7 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 82 kDa.Observed band size : 82 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM5 antibody [EP2683Y]; TA301223 at 1/250 dilution staining MCM5 in human squamous cell cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MCM5 antibody [EP2683Y](TA301223); TA301223 showing positive staining in Normal tonsil tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MCM5 antibody [EP2683Y](TA301223); TA301223 showing negative staining in Normal prostate tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MCM5 antibody [EP2683Y](TA301223); TA301223 showing positive staining in Prostatic carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MCM5 antibody [EP2683Y](TA301223); TA301223 showing positive staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MCM5 antibody [EP2683Y](TA301223); TA301223 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MCM5 antibody [EP2683Y](TA301223); TA301223 showing positive staining in Endometrial carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-MCM5 antibody [EP2683Y](TA301223); TA301223 showing positive staining in Colonic adenocarcinoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - MCM5 antibody [EP2683Y]; TA301223 at 1/100 dilution staining MCM5 in HeLa cells by Immunofluorescence.

 

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