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Home Antibody All anti-MCAM antibodies

Anti-MCAM Antibody EPR3207(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307157
  • Rabbit monoclonal antibody against CD146(clone EPR3207(2))
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416601) , 20ug
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for MCAM (NM_006500)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human CD146 was used as an immunogen.
Clone NameEPR3207(2) IsotypeTissue culture supernatant
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens melanoma cell adhesion molecule (MCAM)
Alternative NameCD146; MUC18
Database LinkNP_006491
FunctionCD146 (melanoma-associated antigen A32 or MUC18) is a transmembrane glycoprotein expressed on endothelial cells and is located at the intercellular junction where it plays a role in cell adhesion, and in the cohesion of the endothelial monolayer. CD146 engagement initiates an outside-in signaling pathway involving the protein tyrosine kinases FYN and FAK as well as Paxillin. In addition, it is a signaling molecule involved in the dynamics of actin cytoskeleton rearrangement (1). CD146 is also found to be associated with tumor progression and the development of metastasis in human malignant melanoma. Expression of CD146 is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage (2).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-CD146 antibody [EPR3207(2)]; All lanes : Anti-CD146 antibody [EPR3207(2)] at 1/1000 dilution.Lane 1 : A375 cell lysate.Lane 2 : Fetal artery lysate.Lane 3 : HUVEC cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 72 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-CD146 antibody [EPR3207(2)]; Immunofluorescent analysis of HeLa cells labelling CD146 with TA307157 at 1/250 dilution.

 

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