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Home Antibody All anti-MB antibodies

Anti-MB Antibody EP3081Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307224
  • Rabbit monoclonal antibody against Myoglobin (clone EPR2581Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417346) , 20ug Explanation
100ul 325 In Stock
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WB(2)
IHC(5)
Assay(1)
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Also for MB (NM_005368)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human myglobin was used as an immunogen.
Clone NameEP3081Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsIHC: 1:250 - 500, IP: 1:40, WB: 1:1000 - 5000,
Predicted MW Explanation 17 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens myoglobin (MB), transcript variant 1
Alternative Namemyoglobgin; PVALB
Database LinkNP_005359
Entrez Gene 4151 Human
Entrez Gene 17189 Mouse
Entrez Gene 59108 Rat
FunctionMyoglobin, an intracellular haemoprotein expressed in the heart and oxidative skeletal myofibres of vertebrates, binds molecular oxygen and may facilitate oxygen transport from erythrocytes to mitochondria, thereby maintaining cellular respiration during periods of high physiological demand (1). Myoglobin may serve a variety of functions in muscular oxygen supply, such as oxygen storage, facilitated O(2) diffusion, and myoglobin-mediated oxidative phosphorylation. Disruption of myoglobin results in the activation of multiple compensatory mechanisms that steepen the pO(2) gradient and reduce the diffusion path length for O(2) between capillary and the mitochondria (2).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Myoglobin antibody [EP3081Y]; Performed under reducing conditions. Predicted band size: 17 kDa.Observed band size: 15 kDa. Exposure time: 5 minutes. This image is courtesy of an anonymous customer review.
WB Image
Western blot - Myoglobin antibody [EP3081Y]; All lanes : Anti-Myoglobin antibody [EP3081Y] at 1/5000 dilution.Lane 1 : Human heart muscle lysate.Lane 2 : Mouse heart muscle lysate.Lane 3 : Rat heart muscle lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 17 kDa.Observed band size : 17 kDa.
Assay Image
Other-Anti-Myoglobin antibody [EP3081Y](TA307224); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myoglobin antibody [EP3081Y]; TA307224, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myoglobin antibody [EP3081Y](TA307224); TA307224 showing positive staining in Skeletal muscle tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myoglobin antibody [EP3081Y](TA307224); TA307224 showing negative staining in Normal brain tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myoglobin antibody [EP3081Y](TA307224); TA307224 showing negative staining in Normal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Myoglobin antibody [EP3081Y](TA307224); TA307224 showing negative staining in Normal liver tissue.

 

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