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Anti-MAPT Antibody E178
Also for MAPT (NM_005910)
|A synthetic phospho-peptide corresponding to residues surrounding Ser396 of human Tau was used as immunogen. The antibody only detects Tau phosphorylated on Serine 396. Predicted to cross-react with bovine, based on sequence homology.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||IHC-P: 1:500 - 1:1000; WB: 1:5000; ICC: 1:250; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens microtubule-associated protein tau (MAPT), transcript variant 2|
|DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; TAU|
|Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Taus association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4). |
MAPK signaling pathway
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Western blot - Anti-Tau antibody [E178]; All lanes : Anti-Tau antibody [E178] at 1/1000 dilution.Lane 1 : Untreated SH-SY5Y cell lysate.Lane 2 : SH-SY5Y cell lysate SH SY5Y cell lysate treated with Oka/CalA. Cells are serum-starved overnight, and then treated with 1nM calyculin A and 500nM Okadaic acid for 2 hours at 37°C.Predicted band size : 79 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178]; Immunohistochemistical detection ofTau antibody [E178] on formaldehyde fixed paraffin-embedded rat tongue sectionsAntigen retrieval step: Heat mediated.Buffer Used: Citric acid pH6. Permeabilization: None.Primary antibody incubated at1/1000 for2 hours@ 21°C inTBS/BSA/azide. Secondary antibody: anti Rabbit IgG Conjugated toBiotin(1/200). A strong pattern of immunostaining which appears to be mostly localised to nerve fibres and their cell bodies (Islet of Langerhans cells are also positive). In submitted image of central area of tongue coloured arrowheads indicate features: red for nerves cut in cross-section (T/S), each brown dot representing a single axon green for what appears to me to be small nerve fibres wrapping around a partial muscle fibre black for a Ganglion containing seven positive nerve cell bodies. Surrounding these are collagen fibres (C), adipocytes (A) and skeletal/striated muscle fibres in L/S ( M- showing blue myocyte nuclei arranged around the periphery of each fibre).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [E178]; Immunohistochemistical detection of Tau antibody [E178]on formaldehyde-fixed paraffin-embedded human salivary gland sections. Antigen retrieval step: Heat mediated.Buffer Used: Citric acid pH6. Permeabilization: No. Blocking step: 1% BSAfor 10 mins@ 21°C.TA300315 incubated at 1/1000 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody: anti rabbit IgG conjugated toBiotin (1/200).NB: An interesting pattern of positivity that seems to be supported by the Human Protein Atlas. Coloured arrowheads in the submitted image indicate features: red for positive serous glands, blue for positive intra-lobular collecting ducts, black for negative mucous glands (there is a serous demilune around this acinus), yellow for intralobular collecting ducts, green for nerve tracks in the interlobular areas, blue for positive interlobular collecting ducts. There appears to be a population of positive nuclei but this may be due to the fact that TA300315 @ 1/1000 gives a positivity that is too strong (should be further diluted).
Immunohistochemistry (Paraffin-embedded sections) - Anti-Tau antibody [E178]; Ab32057, at a dilution of 1/500, staining Tau in paraffin embedded human brain sections by Immunohistochemistry.