Anti-MAPK7 Antibody, clone 3D4

Also for MAPK7 (NM_002749)

OriGene Data

Immunogen	Full length human recombinant protein of human MAPK7 (NP_002740) produced in HEK293T cell.
Clone Name	clone 3D4	Isotype	IgG2a
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:200~500, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens mitogen-activated protein kinase 7 (MAPK7), transcript variant 3
Alternative Name	BMK1; ERK4; ERK5; PRKM7
Database Link	NP_002740
Function	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq].
Related Pathway	EGFR1 Signaling Pathway Focal Adhesion MAPK signaling pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK7 (RC203506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK7.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK7 monoclonal antibody.

IF Image

Anti-MAPK7 mouse monoclonal antibody (TA502150) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7(RC203506).

FC Image

HEK293T cells transfected with either RC203506 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAPK7 antibody(TA502150), and then analyzed by flow cytometry.

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Anti-MAPK7 TrueMAB Antibody clone 3D4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data