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Home Antibody All anti-MAPK3 antibodies

Anti-MAPK3 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323355
  • Anti-MAPK3 (Phospho-Thr202) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)
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Also for MAPK3 (NM_002746)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of threonine 202 (F-L-T(p)-E-Y) derived from Human p44/42 MAP Kinase.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 3 (MAPK3), transcript variant 1
Alternative NameERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
Database LinkNP_002737
Entrez Gene 5595 Human
Entrez Gene 26417 Mouse
Entrez Gene 50689 Rat
FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosismTOR signaling pathwayVascular smooth muscle contractionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 42; 44 kDa . Positive control: 293 cells untreated or treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: untreated 293 cells lysate Lane2: 293 cells treated with PMA for 5 minutes lysate Lane3: 293 cells treated with PMA for 15 minutes lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasmic, Nuclear. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using MAPK3 (Phospho-Thr202) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Cytoplasmic, Nuclear. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using MAPK3 (Phospho-Thr202) antibody at dilution 1/100. (Original magnification:×200)

 

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