OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-MAPK3 antibodies

Anti-MAPK3 PHOSPHO Antibody E337

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303838
  • Rabbit monoclonal antibody against Phospho-Erk1/ERK2 (pT202/pY204)(E337) (phospho-specific)
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)
spacer
Also for MAPK3 (NM_002746)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine
Clone NameE337 IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:500; IHC: 1:50; ICC: 1:250
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification IgG fraction
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 3 (MAPK3), transcript variant 1
Alternative NameERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
Database LinkNP_002737
FunctionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq].
Related Pathway
Adherens junction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway
Melanogenesis
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) [E337] antibody; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 42 , 44 kDa.Exposure time : 16 minutes
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) [E337] antibody; Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti-ERK1(pT202/pY204)/ERK2(pT185/pY187) diluted 1:50
FC Image
Flow Cytometry - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) [E337] antibody; Overlay histogram showing HeLa cells stained with TA303838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title