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Home Antibody All anti-JNK1 antibodies

Anti-JNK1 PHOSPHO Antibody EPR5693

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SKU Description Amount Price Availability*  
TA310637 Rabbit Monoclonal antibody against JNK1 (pT183) / JNK2 (pT183) / JNK3 (pT221) (Phospho-specific) 100ul $325 In Stock
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WB(1)
IHC(1)
FC(1)
Assay(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding threonine 221 of human JNK3 was used as immunogen. The antibody will detect JNK1 (pT183), JNK2(pT183) and JNK3 (pT221).
Clone NameEPR5693 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC, FC Suggested DilutionsFC: 1:100; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant 1
Alternative NameJNK3; JNK3A; p493F12; p54bSAPK; PRKM10; SAPK1b
Database LinkNP_002744
Entrez Gene 5602 Human
Entrez Gene 26414 Mouse
Entrez Gene 25272 Rat
FunctionC-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in the heart and nervous system. JNK3 has been implicated in stress-induced neuronal apoptosis (3).
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayWnt signaling pathwayFocal adhesionToll-like receptor signaling pathwayNOD-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) (phospho T183 + T183 + T221) antibody [EPR5693]; All lanes : Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) antibody [EPR5693] at 1/1000 dilution.Lane 1 : NIH 3T3 cell lysate, untreated .Lane 2 : NIH 3T3 cell lysate, treated with Anisomycin.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution
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Other-Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) antibody [EPR5693](TA310637); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) (phospho T183 + T183 + T221) antibody [EPR5693]; TA310637, at 1/100 dilution staining JNK1+JNK2+JNK3 in paraffin-embedded Human brain tissue, by Immunohistochemistry.
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Flow Cytometry - Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) antibody; Overlay histogram showing HeLa cells stained with TA310637 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
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