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Anti-JNK1 PHOSPHO Antibody EPR5693
Also for JNK1 (NM_002753)
|A synthetic phospho-peptide corresponding to residues surrounding threonine 221 of human JNK3 was used as immunogen. The antibody will detect JNK1 (pT183), JNK2(pT183) and JNK3 (pT221).|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC, FC
||FC: 1:100; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Homo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant 1|
|JNK3; JNK3A; p493F12; p54bSAPK; PRKM10; SAPK1b|
|C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in the heart and nervous system. JNK3 has been implicated in stress-induced neuronal apoptosis (3).|
MAPK signaling pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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Western blot - Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) (phospho T183 + T183 + T221) antibody [EPR5693]; All lanes : Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) antibody [EPR5693] at 1/1000 dilution.Lane 1 : NIH 3T3 cell lysate, untreated .Lane 2 : NIH 3T3 cell lysate, treated with Anisomycin.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution
Other-Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) antibody [EPR5693](ab124956); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) (phospho T183 + T183 + T221) antibody [EPR5693]; ab124956, at 1/100 dilution staining JNK1+JNK2+JNK3 in paraffin-embedded Human brain tissue, by Immunohistochemistry.
Flow Cytometry - Anti-JNK1 + JNK2 + JNK3(phospho T183+T183+T221) antibody [EPR5693]; Overlay histogram showing HeLa cells stained with ab124956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.