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Anti-MAPK1 TRUEMAB Antibody Clone OTI4C2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA500485 anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI4C2 (formerly 4C2) 100ul 325
In Stock
LC408481 MAPK1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500485 Carrier-free (BSA/glycerol-free) MAPK1 mouse monoclonal antibody, clone OTI4C2 (formerly 4C2) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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Also for MAPK1 (NM_138957)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.
Clone NameClone OTI4C2 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:50~100, IP 2ug/500ul
Predicted MW Explanation 41.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 2
Alternative NameERK; ERK-2; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2
Database LinkNP_620407
Entrez Gene 5594 Human
Entrez Gene 116590 Rat
Entrez Gene 698569 Monkey
Entrez Gene 477575 Dog
FunctionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosismTOR signaling pathwayVascular smooth muscle contractionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.

IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500485)

IF Image
Anti-MAPK1 mouse monoclonal antibody (TA500485) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1(RC204703).

IF Image
Immunofluorescent staining of COS7 cells using anti-MAPK1 mouse monoclonal antibody (TA500485).

IP Image
Immunoprecipitation(IP) of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAPK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.


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