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Home Antibody All anti-MAPK1 antibodies

Anti-MAPK1 TRUEMAB Antibody Clone OTI4D10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500469 anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI4D10 (formerly 4D10) 100ul $325 In Stock
LC408481 MAPK1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500469 Carrier-free (BSA/glycerol-free) MAPK1 mouse monoclonal antibody, clone OTI4D10 (formerly 4D10) 100ug $450 3-4 weeks
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WB(2)
IP(1)
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Also for MAPK1 (NM_138957)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.
Clone NameClone OTI4D10 IsotypeIgG2a
Species ReactivityHuman, Dog, Rat, Monkey Concentration1.1 mg/ml
Guaranteed Application *WB, IP Suggested DilutionsWB 1:2000, IP 2-4ug/mg
Predicted MW Explanation 41.4 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 2
Alternative NameERK; ERK-2; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2
Database LinkNP_620407
Entrez Gene 5594 Human
Entrez Gene 116590 Rat
Entrez Gene 698569 Monkey
Entrez Gene 477575 Dog
FunctionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosismTOR signaling pathwayVascular smooth muscle contractionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
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Immunoprecipitation of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibody (Negative control: IP without adding anti-MAPK1 antibody). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant
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