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Anti-MAPK1 PHOSPHO Antibody
Also for MAPK1 (NM_053842)
|Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000, IHC: 1:500
|100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
|Rattus norvegicus mitogen activated protein kinase 1 (Mapk1)|
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Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: Î»-Ptase). The blot is identical to the control except that it was incubated in Î»-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with Î»-Ptase.