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Anti-MAP3K5 Antibody EP553Y


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against MAP3K5 (Clone EP553Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401794) , 20ug Explanation
100ul $325 3-7 Days
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Also for MAP3K5 (NM_005923)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in N-terminus of human MEKK5/ASK1 was used as an immunogen.
Clone NameEP553Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:2000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5)
Alternative NameASK1; MAPKKK5; MEKK5
Database LinkNP_005914
Entrez Gene 4217 Human
Entrez Gene 26408 Mouse
FunctionApoptosis signal-regulating kinase-1 (ASK1, MEKK5, MAPKKK5) is a MAP3k type protein kinase belonging to mitogen-activated protein kinase kinase kinase (MAPKKK) family. As a regulator of JNK and p38 MAPK signaling pathways, ASK1/MEKK5 phosphorylates MMK4 to activate c-Jun N-terminal kinase (JNK) and phosphorylates MEK3 and MKK6 to activate p38. ASK1/MEKK5 is activated by oxidative stress, TNF via TRAF2, Fas via Daxx, calcium overload, and endoplasmic reticulum stress (1). ASK1/MEKK5 is inactivated by Trx, Grx, 14-3-3, and protein serine/threonine phosphatase 5 (2). While elevated level of ASK1/MEKK5 can induce apoptosis, a catalytically inactive ASK1/MEKK5 acts as an inhibitor in TNF-alpha induced apoptosis. Over-expression of ASK1/MEKK5 has also been implicated in cell differentiation, such as in keratinocyte differentiation through p38 pathway (3). ASK1/MEKK5 has been implicated as a therapeutic target for neurodegenerative disorder sand cardiac dysfunction (4)
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayNeurotrophin signaling pathwayAmyotrophic lateral sclerosis (ALS)

* Availability is in business days
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Western blot - ASK1 antibody [EP553Y]; Anti-ASK1 antibody [EP553Y] at 1/2000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-rabbit, HRP labelled at 1/2000 dilution.Predicted band size : 155 kDa.Observed band size : 155 kDa.
Immunohistochemistry (Paraffin-embedded sections) - ASK1 antibody [EP553Y]; Ab45178 (1/10) staining human ASK1 in human lung carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - ASK1 antibody [EP553Y]; Immunofluorescent staining of HeLa cells using TA300688 (1/10).
Flow Cytometry - ASK1 antibody [EP553Y]; Overlay histogram showing HeLa cells stained with TA300688 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.


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