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Home Antibody All anti-MAP3K3 antibodies

Anti-MAP3K3 Antibody ep599y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303594
  • Rabbit monoclonal antibody against MEKK3 (N-term)(EP599Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419352) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
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Also for MAP3K3 (NM_002401)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-term of human MEKK3 was used as immunogen.
Clone Nameep599y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:50 - 1:100; FC: 1:20; IP: 1:20
Predicted MW Explanation 71 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Whole Cell Lysate

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2
Alternative NameMAPKKK3; MEKK3
Database LinkNP_002392
Entrez Gene 4215 Human
Entrez Gene 26406 Mouse
Entrez Gene 303604 Rat
FunctionThis gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayNeurotrophin signaling pathwayGnRH signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MEKK3 antibody [ep599y]; Anti-MEKK3 antibody [ep599y] at 1/1000 dilution + Hela cell lysate at 10 ug.Predicted band size : 71 kDa.Observed band size : 70 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MEKK3 antibody [ep599y]; Immunohistochemical analysis of paraffin embedded human breast adenocarcinoma using TA303594 diluted 1:50.
IF Image
Immunocytochemistry - MEKK3 antibody [ep599y]; Immunofluorescent staining of HeLa cells using TA303594 diluted 1:50.

 

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