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Anti-MAP3K3 Antibody ep599y
Also for MAP3K3 (NM_002401)
|A synthetic peptide corresponding to residues in the N-term of human MEKK3 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:50 - 1:100; FC: 1:20; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Whole Cell Lysate
|Homo sapiens mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2|
Entrez Gene 4215 Human
Entrez Gene 26406 Mouse
Entrez Gene 303604 Rat
|This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]. |
|Protein KinaseDruggable Genome MAPK signaling pathwayNeurotrophin signaling pathwayGnRH signaling pathway|
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Western blot - MEKK3 antibody [ep599y]; Anti-MEKK3 antibody [ep599y] at 1/1000 dilution + Hela cell lysate at 10 ug.Predicted band size : 71 kDa.Observed band size : 70 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MEKK3 antibody [ep599y]; Immunohistochemical analysis of paraffin embedded human breast adenocarcinoma using TA303594 diluted 1:50.
Immunocytochemistry - MEKK3 antibody [ep599y]; Immunofluorescent staining of HeLa cells using TA303594 diluted 1:50.