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Anti-MAP2K3 PHOSPHO Antibody
Also for MAP2K3 (NM_145109)
|The antiserum was produced against synthesized phosphopeptide derived from human MKK3 around the phosphorylation site of Serine 189(V-D-SP-V-A).|
||WB: 1:500-1:3000; IHC: 1:50-1:100; ELISA: 1:5000
|Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Western blot analysis of the extracts from MDA-MB- 435 cells treated or untreated with UV using MKK3 (Ab-189) Antibody and MKK3 (Phospho- Ser189) Antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin- embedded breast carcinoma, using MKK3 (Phospho- Ser189) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.The picture on the right is treated with the synthesized peptide.