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Anti-MAP2K4 Antibody EP615Y
Also for MAP2K4 (NM_003010)
|A synthetic peptide corresponding to residues in the C-term of human MKK4 was used as immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC or IP.
|Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), transcript variant 1|
|JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1|
|MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylate and activate p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4). |
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - SEK1 / MKK4 antibody [EP615Y]; Anti-MEK4 antibody [EP615Y] at 1/1000 dilution + HeLa cell lysate.Predicted band size : 44 kDa.Observed band size : 44 kDa.
Immunohistochemistry - MEK4 antibody [EP615Y]; IHC-P of lung adenocarcinoma using anti-MKK4 (C term) TA300580 at 1/100.
Flow Cytometry - Anti-MEK4 antibody [EP615Y]; Overlay histogram showing K562 cells stained with TA300580 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.