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Home All anti-MAP2K3 antibodies
Anti-MAP2K3 TrueMAB Antibody Clone 5F9
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for MAP2K3 (NM_145109)
OriGene Data
| Full length human recombinant protein of human MAP2K3(NP_659731) produced in HEK293T cell. |
| Clone 5F9 |
IgG2b |
| Human , Dog , Rat , Monkey , Mouse |
0.5~1.0 mg/ml (Lot Dependent) |
| WB, IHC, IF |
WB 1:200~2000, IHC 1:150, IF 1:100, |
| PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose) |
Reference Data
| Homo sapiens mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B |
| MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2 |
| NP_659731 |
| The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]. |
EGFR1 Signaling Pathway Focal Adhesion MAPK signaling pathway Senescence and Autophagy TGF Beta Signaling Pathway Toll-like receptor signaling pathway |
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K3 (RC207115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K3.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAP2K3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAP2K3 mouse monoclonal antibody. (TA505840)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAP2K3 mouse monoclonal antibody. (TA505840)
IF Image
Anti-MAP2K3 mouse monoclonal antibody (TA505840) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K3(RC207115).
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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