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Anti-MAP2K3 TRUEMAB Antibody Clone OTI3C3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA505618 MAP2K3 (MEK3) mouse monoclonal antibody, clone OTI3C3 (formerly 3C3) 100ul 325
In Stock
LC403425 MAP2K3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF505618 Carrier-free (BSA/glycerol-free) MAP2K3 mouse monoclonal antibody, clone OTI3C3 (formerly 3C3) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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Also for MAP2K3 (NM_145109)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human MAP2K(NP_659731) produced in HEK293T cell.
Clone NameClone OTI3C3 IsotypeIgG2a
Species ReactivityHuman , Dog Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested Dilutions WB 1:200~2000, IF 1:100,
Predicted MW Explanation 39.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 3 (MAP2K3), transcript variant B
Alternative NameMAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2
Database LinkNP_659731
Entrez Gene 5606 Human
Entrez Gene 489547 Dog
FunctionThe protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq].
Related PathwayProtein KinaseTranscription FactorsDruggable Genome MAPK signaling pathwayToll-like receptor signaling pathwayFc epsilon RI signaling pathwayGnRH signaling pathwayAmyotrophic lateral sclerosis (ALS)

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K3 (RC207115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K3.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAP2K3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IF Image
Anti-MAP2K3 mouse monoclonal antibody (TA505618) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K3(RC207115).


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