Anti-MAP2K2 Antibody, clone 5F6

Also for MAP2K2 (NM_030662)

OriGene Data

Immunogen	Protein expressed in 293T cell transfected with human MAP2K2 expression vector
Clone Name	clone 5F6	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, FC	Suggested Dilutions	WB 1:2000, IHC 1:150, FLOW 1:100,
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)
Alternative Name	MAPKK2; MEK2; MKK2; PRKMK2
Database Link	NP_109587
Function	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases.Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.
Related Pathway	EGFR1 Signaling Pathway Focal Adhesion MAPK signaling pathway Melanogenesis Toll-like receptor signaling pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K2 (RC201827, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAP2K2 mouse monoclonal antibody. (TA500466)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MAP2K2 mouse monoclonal antibody. (TA500466)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAP2K2 mouse monoclonal antibody. (TA500466)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MAP2K2 mouse monoclonal antibody. (TA500466)

FC Image

HEK293T cells transfected with either RC201827 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MAP2K2 antibody(TA500466), and then analyzed by flow cytometry.

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Anti-MAP2K2 TrueMAB Antibody clone 5F6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data