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Home Antibody All anti-MAP2K2 antibodies

Anti-MAP2K2 PHOSPHO Antibody


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SKU Description Amount Price Availability*  
TA321906 Anti-MAP2K2 (Phospho-Thr394) Rabbit Polyclonal Antibody 100ul 325
2-3 Weeks
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OriGene Data

ImmunogenPeptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
Predicted MW Explanation 44kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)
Alternative NameCFC4; MAPKK2; MEK2; MKK2; PRKMK2
Database LinkNP_109587
Entrez Gene 5605 Human
Entrez Gene 26396 Mouse
Entrez Gene 58960 Rat
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayVascular smooth muscle contractionVEGF signaling pathwayGap junctionToll-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 44 kDa . Positive control: HepG2 and Hela cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: HepG2 cells untreated with UV lysate Lane2: HepG2 cells treated with UV lysate Lane3: Hela cells untreated with UV lysate Lane4: Hela cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

IHC Image
Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using MAP2K2 (Phospho-Thr394) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

IF Image
Predicted cell location: Cytoplasm. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using MAP2K2 (Phospho-Thr394) antibody at dilution 1/100. (Original magnification:×200)


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