Anti-MAP2K2 Antibody Y78

SKU	Description	Amount	Price	Availability*
TA300386	Rabbit Monoclonal Antibody against MAP2K2 (Clone Y78) FREE positive control: HEK293T cell transient overexpression lysate (LC403069) , 20ug	100µl	$325	3-7 Days
TA802519S	Purified GAPDH mouse monoclonal antibody (Loading control), clone 2D9	30ul	$100 $50*	In Stock

* Buy one primary antibody, get 50% off a GAPDH loading control antibody.

WB(1)

IHC(1)

IF(1)

Also for MAP2K2 (NM_030662)

OriGene Data

Immunogen	A synthetic peptide corresponding to residues in N-terminus of human MEK2 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members. Predicted to cross-react with mouse, based on sequence homology.
Clone Name	Y78	Isotype	IgG
Species Reactivity	Human, Rat	Concentration
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB: 1: 10,000; IHC: 1:250-1:500; ICC: 1:250-1:500; FC: 1:40; IP: 1: 100
Buffer	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)
Alternative Name	MAPKK2; MEK2; MKK2; PRKMK2
Database Link	NP_109587
Function	MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).
Related Pathway	EGFR1 Signaling Pathway Focal Adhesion MAPK signaling pathway Melanogenesis Toll-like receptor signaling pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

Western blot analysis on Jurkat cell lysate using anti-MEK2 (N-term); dilution 1:10,000.

IHC Image

Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-MEK2 (N-term).

IF Image

Immunofluorescent staining of HeLa cells using anti-MEK2 (N-term).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

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Anti-MAP2K2 Antibody Y78

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data