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Anti-MAP2K2 Antibody Y78
Also for MAP2K2 (NM_030662)
|A synthetic peptide corresponding to residues in N-terminus of human MEK2 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members. Predicted to cross-react with mouse, based on sequence homology.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: 1:250 - 1:500; WB: 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:100; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)|
|CFC4; MAPKK2; MEK2; MKK2; PRKMK2|
|MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).|
EGFR1 Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - MEK2 antibody [Y78]; Anti-MEK2 antibody [Y78] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 44 kDa.Observed band size : 45 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MEK2 antibody [Y78]; Immunohistochemical analysis of paraffin embedded prostate carcinoma using ab32517 at a dilution of 1/250.
Immunocytochemistry/ Immunofluorescence - MEK2 antibody [Y78]; Immunofluorescent staining of HeLa cells using ab32517 at a dilution of 1/250.
Flow Cytometry - MEK2 antibody [Y78]; Overlay histogram showing HeLa cells stained with ab32517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.