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Anti-MAP2K2 Antibody Y78
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Also for MAP2K2 (NM_030662)
|A synthetic peptide corresponding to residues in N-terminus of human MEK2 was used as immunogen. The antibody does not cross-react with other MAP kinase kinase family members. Predicted to cross-react with mouse, based on sequence homology.|
|WB, IHC, IF
||WB: 1: 10,000; IHC: 1:250-1:500; ICC: 1:250-1:500; FC: 1:40; IP: 1: 100
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2)|
|MAPKK2; MEK2; MKK2; PRKMK2|
|MEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).|
EGFR1 Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot analysis on Jurkat cell lysate using anti-MEK2 (N-term); dilution 1:10,000.
Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-MEK2 (N-term).
Immunofluorescent staining of HeLa cells using anti-MEK2 (N-term).