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Anti-MAP2K1 TRUEMAB Antibody Clone OTI3C1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA506069 MAP2K1 (MEK1) mouse monoclonal antibody, clone OTI3C1 (formerly 3C1) 100ul 325
In Stock
LC400974 MAP2K1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506069 Carrier-free (BSA/glycerol-free) MAP2K1 mouse monoclonal antibody, clone OTI3C1 (formerly 3C1) 100ug $450 3-4 weeks
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Also for MAP2K1 (NM_002755)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human MAP2K1(NP_002746) produced in HEK293T cell.
Clone NameClone OTI3C1 IsotypeIgG2a
Species ReactivityHuman , Dog , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:200~4000, IHC 1:150, IF 1:100,
Predicted MW Explanation 43.3 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Alternative NameCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Database LinkNP_002746
Entrez Gene 5604 Human
Entrez Gene 26395 Mouse
Entrez Gene 710415 Monkey
Entrez Gene 478347 Dog
FunctionThe protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosisVascular smooth muscle contractionDorso-ventral axis formationMore Pathways >>

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* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (RC218460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAP2K1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506069)
Anti-MAP2K1 mouse monoclonal antibody (TA506069) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1(RC218460).


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