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Home Antibody All anti-MAP2K1 antibodies

Anti-MAP2K1 TRUEMAB Antibody Clone OTI1F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500644 anti-MAP2K1 (MEK1) mouse monoclonal antibody, clone OTI1F5 (formerly 1F5) 100ul $325 In Stock
LC400974 MAP2K1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500644 Carrier-free (BSA/glycerol-free) MAP2K1 mouse monoclonal antibody, clone OTI1F5 (formerly 1F5) 100ug $450 3-4 weeks
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WB(1)
IF(1)
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Also for MAP2K1 (NM_002755)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human MAP2K1 expression vector
Clone NameClone OTI1F5 IsotypeIgG1
Species ReactivityHuman Concentration0.99 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB 1:2000, IF 1:100,
Predicted MW Explanation 43.4 KDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Alternative NameCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Database LinkNP_002746
Entrez Gene 5604 Human
FunctionThe protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosisVascular smooth muscle contractionDorso-ventral axis formationMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (RC218460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1 .
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Anti-MAP2K1 mouse monoclonal antibody (TA500644) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1 (RC218460).
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