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Anti-MAP2K1 Antibody E237
Also for MAP2K1 (NM_002755)
|A synthetic phospho-peptide corresponding to residues surrounding Ser218 and Ser222 of human MEK1 was used as immunogen. The antibody only detects MEK1 phosphorylated on Serine 218 and 222.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:500~1000; IHC: 1:50-100; ICC: 1:50-100; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
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Western blot - MEK1 (phospho S218 + S222) antibody [E237]; All lanes : Anti-MEK1 (phospho S218 + S222) antibody [E237] at 1/1000 dilution.Lane 1 : Serum starved A431 cells lysate.Lane 2 : EGF treated serum starved A431 cells. lysate.Predicted band size : 45 kDa.Observed band size : 43 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MEK1 (phospho S218 + S222) antibody [E237]; Ab32088, at a 1/50 dilution, staining MEK1 in paraffin embedded skin carcinoma tissue sections by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S218 + S222) antibody [E237]; TA300417 staining MEK1 (phospho S218 + S222) in SKNSH cells treated with dopamine hydrochloride , by ICC/IF. Increase in MEK1 (phospho S218 + S222) expression correlates with increased concentration of dopamine hydrochloride, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of (dopamine hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300417 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.