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Home Antibody All anti-MAP2K1 antibodies

Anti-MAP2K1 Antibody E237

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300417
  • Rabbit Monoclonal Antibody against MAP2K1 (Clone E237)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400974) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for MAP2K1 (NM_002755)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser218 and Ser222 of human MEK1 was used as immunogen. The antibody only detects MEK1 phosphorylated on Serine 218 and 222.
Clone NameE237 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500~1000; IHC: 1:50-100; ICC: 1:50-100; IP: 1:30
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Alternative NameCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Database LinkNP_002746
FunctionMEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway
Melanogenesis
Senescence and Autophagy
Toll-like receptor signaling pathway

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WB Image
Western blot - MEK1 (phospho S218 + S222) antibody [E237]; All lanes : Anti-MEK1 (phospho S218 + S222) antibody [E237] at 1/1000 dilution.Lane 1 : Serum starved A431 cells lysate.Lane 2 : EGF treated serum starved A431 cells. lysate.Predicted band size : 45 kDa.Observed band size : 43 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MEK1 (phospho S218 + S222) antibody [E237]; Ab32088, at a 1/50 dilution, staining MEK1 in paraffin embedded skin carcinoma tissue sections by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 (phospho S218 + S222) antibody [E237]; TA300417 staining MEK1 (phospho S218 + S222) in SKNSH cells treated with dopamine hydrochloride , by ICC/IF. Increase in MEK1 (phospho S218 + S222) expression correlates with increased concentration of dopamine hydrochloride, as described in literature.The cells were incubated at 37°C for 24h in media containing different concentrations of (dopamine hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300417 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

 

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