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Anti-MAP2 PHOSPHO Antibody EPR2361
Also for MAP2 (NM_031846)
|A phospho-specific peptide corresponding to residues surrounding Serine 136 of human MAP2 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens microtubule-associated protein 2 (MAP2), transcript variant 3|
|MAP2A, MAP2B, MAP2C;DKFZp686I2148; MAP2A; MAP2B; MAP2C; OTTHUMP00000163916; microtubule-associated protein 2|
|Microtubule-associated protein-2 (MAP-2) is a family of heat-stable, phosphoproteins expressed predominantly in the cell body and dendrites of neurons. Three major MAP-2 isoforms, (MAP2a, and MAP2b (280 kDa), MAP2c (75 kDa)) are differentially expressed during the development of the nervous system and have an important role in microtubule dynamics (1). MAP2 is proposed to play important roles in the outgrowth of neuronal processes, synaptic plasticity and neuronal cell death. MAP2 may perform many functions including the nucleation and stabilization of microtubules, the regulation of organelle transport within axons and dendrites, as well as the anchorage of regulatory proteins such as protein kinases which may be important for signal transduction (2). MAP2 is an important cytoskeleton protein whose phosphorylation in response to extracellular signal is involved in the regulation of neurite outgrowth and neuronal plasticity. Findings suggest that epinephrine induces phosphorylation of MAP2c at Serine 136 through an alpha 2-adrenoceptor mediated, ERK/PKC-dependent signaling pathway, which may contribute to the stabilization of neuritis (3). |
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Western blot - MAP2 (phospho S136) antibody [EPR2361]; All lanes : Anti-MAP2 (phospho S136) antibody [EPR2361] at 1/1000 dilution.Lane 1 : Untreated fetal brain lysates.Lane 2 : Fetal brain lysates treated with Alkaline Phosphatase.Lane 3 : Mouse brain lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP/AP polymerized antibody.Predicted band size : 200 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAP2 (phospho S136) antibody [EPR2361]; TA307036, at a 1/100 dilution, staining MAP2 in paraffin embedded Human brain tissue by Immunohistochemical analysis.